Glut4 antibody cell signaling

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August undefined, 2024

WebPolyclonal Antibody for studying Glut4. Cited in 18 publications. Highly specific and rigorously validated in-house, Glut4 Antibody (CST #2299) is ready to ship. Web(2213) Glut4 (1F8) Mouse mAb - Cell Signaling Technology - CiteAb. 188 citations – A mouse monoclonal antibody, raised against Solute carrier family 2, facilitated glucose transporter member 4 (Human), supplied by Cell Signaling Technology. ... Monoclonal antibody is produced by immunizing animals with partially purified intracellular glucose ...

Glut4 Antibody Cell Signaling Technology

WebWestern Blot: Glut4 Antibody [NBP1-49533SS] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in … WebImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab216661) Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat … blvd grub and subs

Need for GLUT4 Activation to Reach Maximum Effect of Insulin …

WebSep 1, 2002 · There is a need to understand whether the amount of GLUT4 at the cell surface determines the extent of glucose uptake in response to insulin. Thus, we created … WebSep 4, 2024 · The GLUT4 antibody, Akt antibody, phospho-Akt (Ser473) antibody, phospho-AMPK antibody, AMPK antibody, and phospho-PKC (pan) (Thr410) antibody were purchased from Cell Signaling Technology (Beverly, MA). The anti-c-myc mouse monoclonal antibody and FITC antibody were both purchased from TransGen Biotech … WebDec 20, 2010 · To test whether there exist other factors in serum that regulate cell surface GLUT4 levels, 3T3-L1 adipocytes were incubated for 20 minutes with 100 nM insulin, … cleveland clinic lab locations akron

Neferine Promotes GLUT4 Expression and Fusion With the Plasma …

WebGLUT4 Antibody (PA5-23052) in ICC/IF. Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase 3T3-L1 cells differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. WebCell lysates were immunoblotted with an anti-GLUT4 antibody, anti-p-GLUT4 antibody, and anti-GAPDH antibody. (A) Panels show representative blot of GLUT4, p-GLUT4 … blvd groupWebInsulin stimulates glucose transport largely by mediating translocation of the insulin-sensitive glucose transporter (GLUT4) from an intracellular compartment to the plasma membrane. Using single cell microinjection of 3T3-L1 adipocytes, coupled with immunofluorescence detection of GLUT4 proteins, we have determined that inhibition of endogenous p21ras … blvd hair co

"WebGlut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin … Monoclonal Antibody for studying Glut4. Cited in 185 publications. Validated for … " - Glut4 antibody cell signaling

Glut4 antibody cell signaling

Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)

WebAll lanes : Anti-Glucose Transporter GLUT4 antibody (ab33780) at 1 µg/ml Lane 1 : Recombinant Protein GLUT4 (Partial, Tagged) at 0.1 µg Lane 2 … WebJul 25, 2024 · In order to assess the effects of n-3PUFA on fatty acid metabolism and insulin sensitivity in muscle cells, Cell Signaling Technology GLUT4 antibody (Cell Signaling Technology, 2213) was used in western blot on mouse samples (fig 1). Biochim Biophys Acta (2016) ncbi. mouse monoclonal (1F8) 2213.

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WebCell Signaling Technology. 3 Trask Lane. Danvers, MA 01923. [email protected]. www.cellsignal.com. 8776162355. headquarters: USA. Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help ... WebGLUT4 (SLC2A4) is the insulin-responding glucose transporter, found predominantly in muscle cells and adipocytes (fat cells). After a meal, glucose that is absorbed from the …

WebGlucose transporter type 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4, is a protein encoded, in humans, by the SLC2A4 … WebAug 6, 2024 · GLUT4 and CD36 cell surface delivery is triggered by insulin- and contraction-induced signaling, which share conserved downstream effectors. ... The cells were then incubated with a combination of primary antibody pairs (anti-Rab8a (Rabbit, Cat# 55296-1-AP, Proteintech, Rosemont, IL, USA), anti-Rab11 (Rabbit, Cat# 5589S, Cell …

WebOct 2, 2001 · It has been recently reported that insulin recruits a novel signaling machinery to lipid rafts required for insulin ... we verified the presence of GLUT4 by direct fluorochrome labeling of polyclonal anti-GLUT4 antibody and by using a biochemical method. Confocal microscopy analysis indicated the codistribution of GLUT4 (examined by Alexa Fluor ... WebSep 4, 2024 · e Co-immunoprecipitation assay for interaction between endogenous PGRMC1 and GLUT4. 3T3L1 cell lysate was incubated with anti-PGRMC1 antibody or normal rabbit IgG, and then incubated with 10 µl ...

WebCell Signaling Technology Inc mouse anti glut4 monoclonal antibody Mouse Anti Glut4 Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various …

WebJun 14, 2013 · Membranes were stained with anti-GLUT4 antibody (C-20 goat polyclonal antibody, Santa Cruz Biotech), total Akt antibody (Cell Signaling), phospho-CREB … blvd hagerstownWebSep 1, 2002 · There is a need to understand whether the amount of GLUT4 at the cell surface determines the extent of glucose uptake in response to insulin. Thus, we created a. ... and anti-phospho-ATF-2 (Thr 71) polyclonal antibody from Cell Signaling Technology (Beverly, MA). A polyclonal anti-GLUT4 rabbit antibody was raised in our laboratory to a ... blvd green the woodlandsWebSep 4, 2024 · The GLUT4 antibody, Akt antibody, phospho-Akt (Ser473) antibody, phospho-AMPK antibody, AMPK antibody, and phospho-PKC (pan) (Thr410) antibody … blvd grill johnstownWebGLUT4 Antibody. Check out specifications and order GLUT4 Antibody online. ... 3T3L1 (2), MCF-7 (4) cell lysates and Mouse heart (3) tissue lysate. Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 mouse mAb with DAB staining. ... Western blot was used to determine the levels of insulin signaling related ... cleveland clinic laboratories billingWebThe Glut4 (1F8) Mouse mAb Antibody from Cell Signaling Technology is a Mouse Monoclonal antibody to SLC2A4. This antibody recognizes Human, Mouse, and Rat … cleveland clinic lab main campusWebGlut4 in L6 Rat Cell Line. Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and ... blvd handicap vehiclesWebThe anti-GLUT4 antibody was obtained from Abcam (Cambridge, UK). The Anti-GAPDH rabbit polyclonal antibody and the high sensitivity chemiluminescence detection kit and BCA protein assay kit were purchased from Kangwei Century Biology Co., Ltd. (Beijing, China). Goat anti-Rabbit IgG was provided by Lianke Bio Company (Hangzhou, China). blvd handicap vans for sale by owner