Genepharma fish
http://www.genepharma.com/products.php WebThe experiment was performed in 4 groups, including cells transfected with NC (negative control of miR‐2834 mimics, GenePharma, Shanghai, China) and pIEX4‐EGFP, ... Subcellular localization of lncR26319 by FISH showed that lncR26319 was present in both the nucleus and cytoplasm, ...
Genepharma fish
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WebNov 18, 2024 · FISH was performed according to the manufacturer’s instruction (GenePharma, Shanghai, China). Translation analysis After 48 h of transfection, treating cells with fresh DMEM containing 10 µg/mL puromycin for 5 min at 37 °C before harvesting. WebShanghai GenePharma rna fish kit Rna Fish Kit, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO …
WebJan 15, 2024 · Section snippets Animal treatment. A total of 60 weanling pigs (Duroc × Landrace × Yorkshire), 30-day-old (a mixture of male and female, approximate 10 kg), were fed with a basic diet for a 7-day adaptation period. WebF-circEA1 was resistant to RNase R treatment. d Sanger sequencing showed the junction site of F-cricEA1. e FISH and f RNA nuclear and cytoplasmic isolation assay showed that …
WebFigure Legend Snippet: LINC00326 is downregulated and associated with favorable prognosis in NSCLC. a Levels of LINC00326 in NSCLC and normal tissues, measured by RT-qPCR and normalized to control values assay (n = 96). b LINC00326 levels measured by FISH. c LINC00326 levels in relation to NSCLC tumor stage (I&II, III&IV), measured …
WebThe FISH experiment was carried out according to the instruction of the RNA FISH kit (GenePharma) and images were photographed by confocal microscopy (Leica Microsystems). All probe sequences used for the FISH assay are summarized in Additional file 1 Table S1. CircRNA pull-down assay.
WebMay 4, 2024 · The primers of ZO-1, occludin, ACTA2 and GAPDH were all designed and synthesized by GenePharma (Shanghai, China). Trans-epithelial electrical resistance (TEER) analysis ... D The occurrence of BT was assessed using EUB338 FISH detection. N = 10. E The mRNA levels of zonula occludens-1 (ZO-1) and occludin were measured by … huf to rWebGene-specific primers for PCR-based applications are commercially available. These ready-to-use oligonucleotide sets usually come in pairs, including the forward and reverse primers. In eliminating the step of manually designing primer sequences for genes of interest, these primers can accelerate molecular experiments in gene expression and in ... huf to pounds sterlingWebAug 25, 2024 · Phosphatidylinositol transfer protein membrane-associated 1 (PITPNM1) contains a highly conserved phosphatidylinositol transfer domain which is involved in phosphoinositide trafficking and signaling transduction under physiological conditions. However, the functional role of PITPNM1 in cancer progression remains unknown. Here, … holiday cottages in rhiwWebGenePharma 295 من المتابعين على LinkedIn. GenePharma is a leading RNAi company in Asia, providing comprehensive products for siRNA and microRNA studies. GenePharma Co., … huf to randWebMay 25, 2024 · Fluorescent in situ hybridization (FISH) The circ-PTK2 probe (5′-CTTTAAACCAACATCTTTTC TGACACAGAGACGGCGTGT-3′) was provided by GenePharma. FISH was conducted (GenePharma), and probe signal on cells was observed under fluorescent microscopy (Carl Zeiss, Jena, Germany). Tumor formation … holiday cottages in rhayaderhttp://en.genepharma.com/en/ holiday cottages in ringstead norfolkWebFish assay. Prepare the probes. Then, a larger wet box was used to place the slices crosswise, and 10-µL probes were dropped on the sliced tissue. Add a coverslip, cover the wet box and incubate at 37°C for 12-16 h. holiday cottages in ringwood