Ctcf kda
WebThe recombinant protein with a predicted molecular weight of 43.3 kDa was removed from the gel and purified by electroelution. The electroelution was conducted in cellulose tubes (Sanko Junyaku Co., Ltd., Tokyo, Japan) filled with an electroelution buffer (100 mM sodium phosphate and 0.1% SDS; pH 7.0) at 100 V and 100 mA for 17 h. WebJun 22, 2016 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human CTCF DNA-binding domain in complex with a known CTCF-binding site. While ZF2 does not make sequence-specific …
Ctcf kda
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WebBPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. WebMay 1, 2024 · The loss of CTCF poly ADP-ribosyltion in breast cancer leading to the expression of both 180-kDa and 130-kDa in comparison to only 180-kDa CTCF in normal breast tissues likely ... 2009). Although CTCF-130-kDa or Rb2/p130 can be used as the biomarker for the cancer progression, the utility of CTCF-130-kDa as the disease …
WebThis 70 kDa band for CTCF has been described previously [197,245] as a possible N-terminally truncated CTCF protein generated by alternative translation of CTCF mRNA which is known to enhance CTCF ... WebChIP results obtained with the Diagenode antibody directed against CTCF. ChIP was performed with the Diagenode antibody against CTCF (cat. No. C15410210) on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control.
WebAbFlex® CTCF recombinant antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of MCF7 chromatin and 4 µg of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to … WebFeb 1, 1997 · CTCF belongs to the Zn finger transcription factors family and binds to the promoter region of c-myc. CTCF is highly conserved between species, ubiquitous and localised in nuclei. The endogenous CTCF migrates as a 130 kDa (CTCF-130) protein on SDS-PAGE, however, the open reading frame (ORF) of the CTCF cDNA encodes only a …
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WebJan 30, 2024 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human CTCF DNA-binding domain in complex with a known CTCF-binding site. While ZF2 does not make sequence-specific … small world automotiveWebMar 3, 2024 · The shorter CTCF forms are expected to lack several domains; in the ~ 120 kDa form, we estimate that most of the N-terminal part that is responsible for cohesin retention and formation of genomic loops (Pugacheva et al. 2024) is missing, but all eleven zinc-finger still exist. small world aviationWebDec 12, 2024 · Through binding of CTCF binding sites (CBS), CTCF mediates nuclear functions including DNA looping, regulation of gene expression, and RNA splicing. ... only two bands at 110 kDa and 130 kDa were ... hilanddairy.com/hr-formsWebJun 5, 2015 · CCCTC-binding factor (CTCF) is an 82-kDa protein with 11 zinc fingers. 1 It was first identified as a transcriptional repressor of the … hilanddairy.com/holidaysWebBackground Information. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. small world auto reviewsWebFamily First is an opportunity to provide enhanced support to children and families. It aims to prevent foster care placements through the provision of mental health and substance … small world babyWebJun 22, 2016 · PubMed Abstract: The multidomain CCCTC-binding factor (CTCF), containing a tandem array of 11 zinc fingers (ZFs), modulates the three-dimensional organization of chromatin. We crystallized the human … hilandar foundation